n.BA.BT.PrMibi.21HS (Microbiology Practical) 
Module: Microbiology Practical
This information was generated on: 30 November 2023
Microbiology Practical


Version: 3.0 start 01 August 2022


Study Programme Biotechnology
Regulations Applicable RPO, 29 January 2008, School of Life Sciences and Facility Management Academic Regulations, 15 Dec. 2009, Annex for the Bachelor of Biotechnology degree programme
Module Type  
X Compulsory Module    Elective Module    Optional Module
Planned Semester 2nd Semester
Module Coordinator Gottfried Dasen
Telephone / E-Mail +41 (0)58 934 55 67 / gottfried.dasen@zhaw.ch
Gottfried Dasen, Giovanna Spielmann, David Frasson, Rolf Warthmann, Martin Kühni
Entrance Requirements Microbiology 1
Learning Outcomes and Competencies Students will
  • master basic microbiological techniques, including working in a sterile environment.
  • master the preparation and testing of sterile culture media and instruments.
  • be able to cultivate, preserve and identify bacteria and fungi.
  • learn about the quality controls required for microbiological analyses.
  • be able to plan, perform and evaluate growth experiments in shake flasks.
Module Content Biosafety
Basic techniques:
  • Sterile work in the laboratory
  • Preparation and testing of sterile culture media and materials
  • Cultivation of microorganisms (anaerobic and aerobic)
  • Culture determination of bacterial count
  • Use of selective and elective media
  • Preservation of microorganisms
  • Use of the microbiological safety cabinet
Quality control:
  • Testing of culture media
Hygiene monitoring:
  • Measurement of microbial contamination in the air and on surfaces in operating rooms
Bacteria and fungi microscopy:
  • Basic principles of the light microscope
  • Contrasting methods, incl. staining
  • Quantitative microscopy
Environmental biotechnology:
  • Light microscopy and flow cytometry for microbiological analysis of drinking and wastewater.
  • Measurement of aerobic degradation with an O2 electrode (respirometry) and measurement of aerobic degradation of an organic substance according to OECD by means of a BOD5 test (biochemical oxygen demand)
Identification of bacteria and fungi:
  • phenotypic differentiation
  • Species level identification methods (Maldi TOF, 16S rRNA sequencing, biochemical methods, fatty acids)
Growth of microorganisms:
  • Growth experiments in shake flasks with different microorganisms (Escherichia coli, Vibrio natriegens, Lactobacillus plantarum, etc.). Measurement of total cell count, live cell count, substrate utilisation (diauxia), optical density, pH
Follow-up Modules Microbiology 2
Methods of Instruction  Laboratory Practical
Digital Resources Moodle
Lesson Structure / Workload  
 Contact Hours -
 Guided Self-Study 78
 Independent Self-Study 12
 Total Workload 90
Classroom Attendance -
Course work 100%: grade from group work
Language of Instruction  German
Comments The Microbiology Practical module is part of the Practicals 2 module group.



Course: Microbiology Practical
Microbiology Practical


  • No module description is available in the system for the cut-off date of 30 November 2023.