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n.BA.BT.PrMibi.21HS (Microbiology Practical)
Module: Microbiology Practical
This information was generated on: 08 June 2023
No.
n.BA.BT.PrMibi.21HS
Title
Microbiology Practical
Credits
3
Description
Version: 3.0 start 01 August 2022
Study Programme
Biotechnology
Regulations Applicable
RPO, 29 January 2008, School of Life Sciences and Facility Management Academic Regulations, 15 Dec. 2009, Annex for the Bachelor of Biotechnology degree programme
Module Type
X
Compulsory Module
Elective Module
Optional Module
Planned Semester
2nd Semester
Module Coordinator
Gottfried Dasen
Telephone / E-Mail
+41 (0)58 934 55 67 /
gottfried.dasen
@zhaw.ch
Lecturer(s),
Speaker(s),
Associate(s)
Gottfried Dasen, Giovanna Spielmann, David Frasson, Rolf Warthmann, Martin Kühni
Entrance Requirements
Microbiology 1
Learning Outcomes and Competencies
Students will
master basic microbiological techniques, including working in a sterile environment.
master the preparation and testing of sterile culture media and instruments.
be able to cultivate, preserve and identify bacteria and fungi.
learn about the quality controls required for microbiological analyses.
be able to plan, perform and evaluate growth experiments in shake flasks.
Module Content
Biosafety
Basic techniques:
Sterile work in the laboratory
Preparation and testing of sterile culture media and materials
Cultivation of microorganisms (anaerobic and aerobic)
Culture determination of bacterial count
Use of selective and elective media
Preservation of microorganisms
Use of the microbiological safety cabinet
Quality control:
Testing of culture media
Hygiene monitoring:
Measurement of microbial contamination in the air and on surfaces in operating rooms
Bacteria and fungi microscopy:
Basic principles of the light microscope
Contrasting methods, incl. staining
Quantitative microscopy
Environmental biotechnology:
Light microscopy and flow cytometry for microbiological analysis of drinking and wastewater.
Measurement of aerobic degradation with an O2 electrode (respirometry) and measurement of aerobic degradation of an organic substance according to OECD by means of a BOD5 test (biochemical oxygen demand)
Identification of bacteria and fungi:
phenotypic differentiation
Species level identification methods (Maldi TOF, 16S rRNA sequencing, biochemical methods, fatty acids)
Growth of microorganisms:
Growth experiments in shake flasks with different microorganisms (Escherichia coli, Vibrio natriegens, Lactobacillus plantarum, etc.). Measurement of total cell count, live cell count, substrate utilisation (diauxia), optical density, pH
Follow-up Modules
Microbiology 2
Methods of Instruction
Laboratory Practical
Digital Resources
Moodle
Lesson Structure / Workload
Contact Hours
-
Guided Self-Study
78
Independent Self-Study
12
Total Workload
90
Classroom Attendance
-
Assessment
Course work 100%:
grade from group work
Language of Instruction
German
Comments
The Microbiology Practical module is part of the Practicals 2 module group.
Note
Additional available versions:
2.0 start 01 August 2021
Course: Microbiology Practical
No.
n.BA.BT.PrMibi.21HS.P
Title
Microbiology Practical
Note
No module description is available in the system for the cut-off date of 08 June 2023.