EventoWeb
Zürcher Hochschule für Angewandte Wissenschaften
[
German (Switzerland)
German (Switzerland)
] [
English
English
]
Not registered
[home]
[Login]
[Print]
Navigation
Kontakt zu Service Desk
Online-Dokumentation
Allgemeiner Zugriff
Module suchen
n.BA.BT.MB3I2.23HS (Molecular Biology 3 and Immunology 2)
Module: Molecular Biology 3 and Immunology 2
This information was generated on: 21 September 2024
No.
n.BA.BT.MB3I2.23HS
Title
Molecular Biology 3 and Immunology 2
Credits
2
Description
Version: 2.0 start 01 August 2024
Study Programme
Biotechnology
Regulations Applicable
RPO, 29 January 2008, School of Life Sciences and Facility Management Academic Regulations, 15 Dec. 2009, Annex for the Bachelor of Biotechnology degree programme
Module Type
X
Compulsory Module
Elective Module
Optional Module
Planned Semester
5th Semester
Module Coordinator
Mathias Schmelcher
Telephone / E-Mail
+41 (0)58 934 57 49 /
mathias.schmelcher@zhaw.ch
Lecturer(s),
Speaker(s),
Associate(s)
Mathias Schmelcher und weitere ReferentInnen
Entrance Requirements
Completion of Molecular Biology 2 and Immunology 1
Learning Outcomes and Competencies
The CRISPR-Cas9 method has made it possible to precisely modify genes within the genome. Using examples, CRISPR-Cas9 as a tool for targeted genome editing will be explained through specific examples.
Students will learn how to use the CRISP-Cas9 method to insert targeted mutations into the genomes of human and animal cells. They will become competent in designing the sgRNA and in developing strategies to detect the mutations as well as analysing the effects caused by this technique. Students will understand how T-cells are reprogrammed with chimeric antigen receptors (CAR-T cells) for the recognition of tumor antigens (such as CD19). They will learn the conditions under which immune responses are misdirected (autoimmune responses), and how antibody and cell-based immunotherapies are used effectively.
Module Content
Explanation and potential of the CRISPR-Cas9 technique through examples
Design of the sgRNA that guides the Cas9 endonuclease to the target DNA and application of CRISPR/Cas tools to determine the optimal sequence to avoid off-target effects
NHEJ and HDR repair mechanisms following DNA cutting by Cas9
Pathology of the immune system
Immunotherapeutic methods: Development of CRISPR-Cas9 strategies that enhance the function of T cells in the immune response
Antibody-based immunotherapies
Follow-up Modules
-
Methods of Instruction
Lectures and tasks
Digital Resources
Videos
Lesson Structure / Workload
Contact Hours
20
Guided Self-Study
8
Independent Self-Study
32
Total Workload
60
Classroom Attendance
No
Assessment
Written exam at the end of the semester 100%
If there is a low number of participants, the lecturer may change the form of a repeat examination after consultation with the head of the study programme: e.g. an oral examination can be used to replace a written one. Please report any changes to the form of examinations by e-mail to
pruefungsadmin.lsfm@zhaw.ch
and Cc. Head of study programme.
Language of Instruction
German
Comments
Cellular and Molecular Immunology, Abul K. Abbas, Andrew H. Lichtman, Elsevier, 10. Edition (5. Juli 2021)
Jiang F, Doudna JA. CRISPR-Cas9 Structures and Mechanisms. Annu Rev Biophys. 2017 May 22;46:505-529. doi: 10.1146/annurev-biophys-062215-010822.
Zhu Y. Advances in CRISPR/Cas9. Biomed Res Int. 2022 Sep 23;2022:9978571. doi: 10.1155/2022/9978571.
Agarwal S, Wellhausen N, Levine BL, June CH. Production of Human CRISPR-Engineered CAR-T Cells. J Vis Exp. 2021 Mar 15;(169). doi: 10.3791/62299.
Note
Additional available versions:
1.0 start 01 August 2023
Course: Molecular Biology 3 and Immunology 2
No.
n.BA.BT.MB3I2.23HS.V
Title
Molecular Biology 3 and Immunology 2
Note
No module description is available in the system for the cut-off date of 21 September 2024.