n.BA.BT.PrZT2.23HS (Cell Culture Technology 2 Practical) 
Module: Cell Culture Technology 2 Practical
This information was generated on: 21 May 2024
No.
n.BA.BT.PrZT2.23HS
Title
Cell Culture Technology 2 Practical
Credits
3

Description

Version: 1.0 start 01 August 2023

 

Study Programme Biotechnology
Regulations Applicable RPO, 29 January 2008, School of Life Sciences and Facility Management Academic Regulations, 15 Dec. 2009, Annex for the Bachelor of Biotechnology degree programme
Module Type  
X Compulsory Module    Elective Module    Optional Module
Planned Semester 5th Semester
Module Coordinator Regine Eibl
Telephone / E-Mail +41 (0)58 934 57 13 / regine.eibl@zhaw.ch
Lecturer(s),
Speaker(s),
Associate(s)		 Regine Eibl and various internal Lecturers
Entrance Requirements Cell Culture Technology 2, Bioprocess Engineering 2
Learning Outcomes and Competencies The aim of the practical is to learn the skills to plan and implement a CHO cell-based mAb production in fed-batch mode in benchtop bioreactors (well-mixed and agitated). Participants will harvest and purify the product (IgG), and present the experimental results per group in a protocol, compare them with data from the literature and also perform a cost analysis of the experiment. The experiment is carried out over a period of 14 days and is to be conducted as a block course of half a day each. For safety reasons, a maximum of 12 students per course can work in the laboratory.
Module Content
  • Inoculum production in shake flasks and in the CL 1000
  • Preparation of the bioreactors
  • Inoculation of the bioreactors
  • Feeding of the bioreactors
  • Harvesting of the bioreactors and product purification
  • Experimental data acquisition (growth and product formation curves, substrate and metabolite profiles) and troubleshooting
  • Literature search to evaluate the experimental results
  • Evaluation and logging of data
Follow-up Modules None
Methods of Instruction  Research, presentations, logging of data
Digital Resources Videos
Lesson Structure / Workload  
 Contact Hours 52
 Guided Self-Study 6
 Independent Self-Study 32
 Total Workload 90
Classroom Attendance Yes
Assessment Coursework 100%: Each group (2, max. 3 students) must submit a report for grading. In the event of unexcused absence from the practical, a technical paper on the topic of the practical (approx. 10 pages) must be written instead and submitted for grading (grade must be ≥ 4).
Language of Instruction  German
Comments The module Practical Cell Culture Technology 2 is part of the Bioprocess Development and Bioengineering module group.

Bibliography

Jagschies et al. (2018) Biopharmaceutical processing: Development, design, and implementation of manufacturing processes. Elsevier ISBN: 978-0081006238

Müller et al. (2022) Intensified and continuous mAb production with single-use systems in Cell Culture Engineering and Technology (ed. Pörtner, R.)
ISBN 978-3-030-79870-3

Müller et al. (2022) Process intensification using a one-step inoculum production and high-seeded fed-batch processes. Chemie Ingenieur Technik. DOI: 10.1002/cite.202200097

 

Course: Cell Culture Technology 2 Practical
No.
n.BA.BT.PrZT2.23HS.P
Title
Cell Culture Technology 2 Practical

Note

  • No module description is available in the system for the cut-off date of 21 May 2024.