n.BA.BT.MB3I2.23HS (Molecular Biology 3 and Immunology 2) 
Module: Molecular Biology 3 and Immunology 2
This information was generated on: 17 May 2024
No.
n.BA.BT.MB3I2.23HS
Title
Molecular Biology 3 and Immunology 2
Credits
2

Description

Version: 1.0 start 01 August 2023

 

Study Programme Biotechnology
Regulations Applicable RPO, 29 January 2008, School of Life Sciences and Facility Management Academic Regulations, 15 Dec. 2009, Annex for the Bachelor of Biotechnology degree programme
Module Type  
X Compulsory Module    Elective Module    Optional Module
Planned Semester 5th Semester
Module Coordinator Martin Sievers
Telephone / E-Mail +41 (0)58 934 57 16 / martin.sievers@zhaw.ch
Lecturer(s),
Speaker(s),
Associate(s)		 Martin Sievers, Jack Rohrer, Steffi Lehmann
Entrance Requirements Completion of Molecular Biology 2 and Immunology 1
Learning Outcomes and Competencies The CRISPR-Cas9 method has made it possible to precisely modify genes within the genome. Using examples, CRISPR-Cas9 as a tool for targeted genome editing will be explained through specific examples.
Students will learn how to use the CRISP-Cas9 method to insert targeted mutations into the genomes of human and animal cells. They will become competent in designing the sgRNA and in developing strategies to detect the mutations as well as analysing the effects caused by this technique. Students will understand how T-cells are reprogrammed with chimeric antigen receptors (CAR-T cells) for the recognition of tumor antigens (such as CD19). They will learn the conditions under which immune responses are misdirected (autoimmune responses), and how antibody and cell-based immunotherapies are used effectively.
Module Content
  • Explanation and potential of the CRISPR-Cas9 technique through examples
  • Design of the sgRNA that guides the Cas9 endonuclease to the target DNA and application of CRISPR/Cas tools to determine the optimal sequence to avoid off-target effects
  • NHEJ and HDR repair mechanisms following DNA cutting by Cas9
  • Pathology of the immune system
  • Immunotherapeutic methods: Development of CRISPR-Cas9 strategies that enhance the function of T cells in the immune response
  • Antibody-based immunotherapies
Follow-up Modules -
Methods of Instruction  Lectures and tasks
Digital Resources Videos
Lesson Structure / Workload  
 Contact Hours 20
 Guided Self-Study 8
 Independent Self-Study 32
 Total Workload 60
Classroom Attendance No
Assessment Written exam at the end of the semester 100%

If there is a low number of participants, the lecturer may change the form of a repeat examination after consultation with the head of the study programme: e.g. an oral examination can be used to replace a written one. Please report any changes to the form of examinations by e-mail to pruefungsadmin.lsfm@zhaw.ch and Cc. Head of study programme.
Language of Instruction  German
Comments Cellular and Molecular Immunology, Abul K. Abbas, Andrew H. Lichtman, Elsevier, 10. Edition (5. Juli 2021)

Jiang F, Doudna JA. CRISPR-Cas9 Structures and Mechanisms. Annu Rev Biophys. 2017 May 22;46:505-529. doi: 10.1146/annurev-biophys-062215-010822.

Zhu Y. Advances in CRISPR/Cas9. Biomed Res Int. 2022 Sep 23;2022:9978571. doi: 10.1155/2022/9978571.

Agarwal S, Wellhausen N, Levine BL, June CH. Production of Human CRISPR-Engineered CAR-T Cells. J Vis Exp. 2021 Mar 15;(169). doi: 10.3791/62299.

 

Course: Molecular Biology 3 and Immunology 2
No.
n.BA.BT.MB3I2.23HS.V
Title
Molecular Biology 3 and Immunology 2

Note

  • No module description is available in the system for the cut-off date of 17 May 2024.