n.BA.BT.PrMB3I2.23HS (Molecular Biology 3 and Immunology 2 Practical) 
Module: Molecular Biology 3 and Immunology 2 Practical
This information was generated on: 17 May 2024
No.
n.BA.BT.PrMB3I2.23HS
Title
Molecular Biology 3 and Immunology 2 Practical
Credits
3

Description

Version: 1.0 start 01 August 2023

 

Study Programme Biotechnology
Regulations Applicable RPO, 29 January 2008, School of Life Sciences and Facility Management Academic Regulations, 15 Dec. 2009, Annex for the Bachelor of Biotechnology degree programme
Module Type  
X Compulsory Module    Elective Module    Optional Module
Planned Semester 5th Semester
Module Coordinator Martin Sievers
Telephone / E-Mail +41 (0)58 934 57 16 / martin.sievers@zhaw.ch
Lecturer(s),
Speaker(s),
Associate(s)		 Martin Sievers and various internal lecturers
Entrance Requirements Completion of Molecularbiology 2 and Immunology 1
Learning Outcomes and Competencies Students will acquire the skills to develop a complete cloning strategy for the production of a partially-humanised monoclonal antibody. They will learn about the practical implementation for antibody production in CHO cells, including their purification and verification of functionality. The skills acquired help students to apply the corresponding monoclonal antibodies which bind specifically and with high affinity to antigens, in both diagnostics and in therapy.
Module Content
  • Creation of a complete cloning strategy to produce a monoclonal partially humanised IgG1 antibody secreted by CHO freestyle cells
  • Application of RT-PCR strategies to amplify the VL and VH domains of the anti-p39 antibody, starting from the isolated RNA of a mouse hybridoma cell line and DNA ligation into the corresponding DNA vectors, providing both human CL and CH1-CH2-CH3 regions
  • Determination of cell viability and number of CHO freestyle cells as well as transient transfection with the corresponding vectors encoding the light and heavy chains
  • Purification of the secreted antibody from a stable transgenic CHO clone via protein A affinity
  • Purification of the recombinant p39 protein of Borrelia burgdorferi from E. coli BL21(DE3) recognised by the produced monoclonal antibody
  • Determination of binding of the produced monoclonal antibody to the p39 antigen- via ELISA, immunofluorescence and biolayer interferometry
Follow-up Modules -
Methods of Instruction  Lectures and tasks
Digital Resources Videos
Lesson Structure / Workload  
 Contact Hours 42
 Guided Self-Study 8
 Independent Self-Study 40
 Total Workload 90
Classroom Attendance Yes
Assessment Written exam at the end of the semester 100%

If there is a low number of participants, the lecturer may change the form of a repeat examination after consultation with the head of the study programme: e.g. an oral examination can be used to replace a written one. Please report any changes to the form of examinations by e-mail to pruefungsadmin.lsfm@zhaw.ch and Cc. Head of study programme.
Language of Instruction  German
Comments Class handouts:
Folien "Molekularbiologie 2 und Immunologie 1“

Selected further reading:

Cellular and Molecular Immunology, Abul K. Abbas, Andrew H. Lichtman, Elsevier, 10. Edition (5. Juli 2021)

Janeway, Immunologie, von Kenneth Murphy, Casey Weaver, 2018, SpringerSpektrum

Sharker SM, Rahman A. A Review on the Current Methods of Chinese Hamster Ovary (CHO) Cells Cultivation for the Production of Therapeutic Protein. Curr Drug Discov Technol. 2021;18(3):354-364. doi: 10.2174/1570163817666200312102137.

 

Course: Molecular Biology 3 and Immunology 2 Practical
No.
n.BA.BT.PrMB3I2.23HS.P
Title
Molecular Biology 3 and Immunology 2 Practical

Note

  • No module description is available in the system for the cut-off date of 17 May 2024.