n.BA.BT.PrZB2TE.23HS (Cell Biology 2 Practical and Tissue Engineering) 
Module: Cell Biology 2 Practical and Tissue Engineering
This information was generated on: 21 May 2024
No.
n.BA.BT.PrZB2TE.23HS
Title
Cell Biology 2 Practical and Tissue Engineering
Credits
3

Description

Version: 1.0 start 01 August 2023

 

Study Programme Biotechnology
Regulations Applicable RPO, 29 January 2008, School of Life Sciences and Facility Management Academic Regulations, 15 Dec. 2009, Annex for the Bachelor of Biotechnology degree programme
Module Type  
X Compulsory Module    Elective Module    Optional Module
Planned Semester 5th Semester
Module Coordinator Jack Rohrer
Telephone / E-Mail +41 (0)58 934 57 17 / jack.rohrer@zhaw.ch
Lecturer(s),
Speaker(s),
Associate(s)		 Jack Rohrer, Leopold von Balthazar, Arezoo Daryadel
Entrance Requirements Completion of Cell Biology 2, Cell Biology Practical and Cell Culture Technology 1
Learning Outcomes and Competencies Students will acquire the ability to independently plan and perform basic techniques for the establishment, maintenance and propagation of cell and tissue cultures. In addition to aseptic methods, attention is paid to compliance with Good Laboratory Practice.
Module Content 6 complex experiments:
  • Creation of a primary culture: the goal is to isolate single cells from a pig kidney by means of explants, to allow them to grow in differently coated wells and after a few days to observe which coating was the most effective or which specific type of cells could be isolated.
  • Inflammation simulation: with LPS, an inflammatory reaction is triggered in the cells (cell reacts by releasing various cytokines), which should be inhibited with different substances in various concentrations. The detection is carried out using a sandwich ELISA in which the concentrations (in ng/ml!) of the cytokines are measured.
  • Cytotoxicity: Various substances with different concentrations will be examined for their toxic influence on HeLa- or MDCK-cells after a period of 24 hrs. The conversion of the MTT substance in the mitochondria of the living cells is also measured and a dose-response curve can be plotted. These data can then be used to determine the IC50 value.
  • 3-D Cultivation – ‘Hanging Drops’: Multicellular spheroids  are grown in hanging drops which serve as an in-vitro model to mimic mini-metastases and areas of solid tumours in vivo. After the delicate transfer of the spheroids to coated and uncoated wells of a well plate, the size and structure of the cells are observed and compared under the microscope two days later.
  • Fluorescence immunostaining: several intracellular structures (microtubules, DNA and Golgi apparatus) of HeLa cells are stained by indirect immunofluorescence (IF), direct staining with a fluorescent dye or expression of a fluorescent protein and viewed under a confocal laser microscope. The aim is to detect as many different stages of mitosis as possible. In addition, we will test how the addition of nocodazole affects the stained cell components and mitosis.
  • Cell differentiation: RANKL (Receptor Activator of NF-κB Ligand) induces differentiation into osteoclasts in RAW cells. The morphological effects on the cells and the rate of differentiation will be assessed microscopically. In addition, osteoclast activity will be determined by detecting TRAP (Tartrate-resistant acidic phosphatase) in the medium of the cultures.
Follow-up Modules None
Methods of Instruction  Practical, self-study
Digital Resources Learning videos
Lesson Structure / Workload  
 Contact Hours 52
 Guided Self-Study 6
 Independent Self-Study 32
 Total Workload 90
Classroom Attendance -
Assessment Coursework (practical report) 100%: Work is completed in groups of 2, reports are submitted and evaluated per group.
Language of Instruction  German
Comments -

 

Course: Cell Biology 2 Practical and Tissue Engineering
No.
n.BA.BT.PrZB2TE.23HS.P
Title
Cell Biology 2 Practical and Tissue Engineering

Note

  • No module description is available in the system for the cut-off date of 21 May 2024.